Probing Protein Conformations

Over the last decade, numerous papers have reported IMS-MS investigations of biomolecules, especially proteins. In these studies, IMS-MS is used to probe the protein conformations as well as the composition, stoichiometry, and arrangement of units in biomolecular complexes. Yet, the majority of these studies were conducted either on drift-tube IMS instruments that do not operate under low-field conditions or on travelling wave IMS (TWAVE or TWIMS) instruments. Use of such instruments can lead to artifacts due to collisionally-induced heating and imprecise measurements of collision cross sections.

The TOFWERK IMS-TOF is a drift-tube based IMS-MS instrument that operates under low-field conditions with a high pressure- and temperature-controlled IMS cell. This ensures that native conditions are preserved during the ion mobility separation. Additionally, the IMS-TOF exhibits superior ion mobility resolution, thus allowing deeper insight into the conformational diversity of biomolecules.