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The high resolution of the IMS-TOF enables confident analysis of isomers, protein conformers, and lipids

Atmospheric-pressure, drift-tube ion mobility spectrometry (IMS) with high-speed TOFMS detection. Fast, decisive 2-dimensional separations with IMS resolving power up to 200.

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Advantages of the IMS-TOF

  • Separate isomers with IMS resolving power of up to 200
  • Measure accurate and reproducible collision cross sections (CCS) without calibration
  • Resolve and identify protein and peptide conformers
  • Analyze unknowns based on CCS, accurate mass, and CID
  • Improve sensitivity 200x compared to conventional DTIMS using artifact-free multiplexing


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The IMS-TOF utilizes a drift-tube ion mobility spectrometer (DTIMS) coupled with a high-speed TOF mass spectrometer.

IMS Resolving Power Up to 200
 IMS Pressure  800 – 1400 mbar ± 1 mbar
 IMS Temperature  ambient – 150°C ± 1°C
 Mass Resolving Power  up to 10000
 Relative Mass Accuracy  2 ppm (internal calibration)
 Sensitivity  150 counts/fmol reserpine
 Dimensions  120 x 60 x 90 cm (bench-top)

 

Drift Tube IMS Cell

  • Use any drift gas in the the pressure and temperature-controlled IMS cell.
  • DTIMS is uniquely capable of direct CCS determination
  • Precisely controlled drift-tube pressure and temperature ensures reproducible CCS measurements

Design

  • Compact, robust design for laboratory and field applications
  • Simple LC coupling with our flexible hardware, software and data format
  • Separation under high-pressure, low-field conditions preserves native structure of protein and supramolecular assemblies
  • IMS resolving power of 200 enables separation of isomers with CCS differences as low as 0.5%
  • Post-IMS CID for precursor and fragment correlation
  • Multiplexed IMS boosts the duty cycle from <1% to 50%, eliminating the conventional trade-offs between sensitivity, mobility range, and resolving power
  • Gas-tight ESI source reduces background signals and harmonizes with drift tube pressure

Software

  • Easy-to-use software for data acquisition and post-processing, based on our open-source, multi-dimensional file format (HDF5)
  • Proprietary data processing yields real gains in signal/noise and IMS resolution

IMS-TOF Publications

2017

  1. Gloess, A., et al., On-line analysis of coffee roasting with ion mobility spectrometry-mass spectrometry (IMS-MS), International Journal of Mass Spectrometry, 2017.  DOI:10.1016/j.ijms.2017.11.017
  2. Grössl, M. & Kornél Nagy, Benefits of ion mobility for analysing monochloropropane-diol esters, Food Additives & Contaminants: Part A, 2017. DOI:10.1080/19440049.2017.1325014
  3. Kiesilä, A., et al., Simultaneous endo and exo Complex Formation of Pyridine[4]arene Dimers with Neutral and Anionic Guests, Angewandte Chemie, 2017. DOI: 10.1002/anie.201704054
  4. Zhang, X., et al., Highly Oxygenated Multifunctional Compounds in α-Pinene Secondary Organic Aerosol, Environ. Science and Technology, 2017. DOI: 10.1021/acs.est.6b06588
  5. Sullivan, M., et al., The metalation of hen egg white lysozyme impacts protein stability as shown by ion mobility mass spectrometry, differential scanning calorimetry, and X-ray crystallography, Chemical Comm., 2017. DOI: 10.1039/c6cc10150j

2016

  1. Lemmnitzer, K., et al., Comparison of ion mobility-mass spectrometry and pulsed-field gradient nuclear magnetic resonance spectroscopy for the differentiation of chondroitin sulfate isomers, Analytical Methods, 2016. DOI: 10.1039/c6ay02531e
  2. Zhang, X., et al., A novel framework for molecular characterization of atmospherically relevant organic compounds based on collision cross section and mass-to-charge ratio, AMT, 2016. DOI:10.5194/acp-16-12945-2016
  3. Krechmer, J., et al., Ion mobility spectrometry–mass spectrometry (IMS–MS) for on- and offline analysis of atmospheric gas and aerosol species, AMT, 2016. In Focus | :10.5194/amt-9-3245-2016
  4. Liu, W., et al., Multidimensional Separation of Natural Products Using Liquid Chromatography Coupled to Hadamard Transform Ion Mobility Mass Spectrometry,  J. Am. Soc. Mass Spec., 2016 DOI: 10.1007/s13361-016-1346-8

2015

  1. Jurcek, O., et al., Superchiral Pd3L6 Coordination Complex and Its Reversible Structural Conversion into Pd3L3Cl6 Metallocycles, Angewandte Chemie, 2015 In Focus | DOI: 10.1002/anie.201506539
  2. Groessl, M., et al., High resolution ion mobility-mass spectrometry for separation and identification of isomeric lipids,  Analyst, 2015, In Focus | DOI: 10.1039/C5AN00838G
  3. Bonakdarzadeh, P., et al., DOSY NMR, X‑ray Structural and Ion-Mobility Mass Spectrometric Studies on Electron-Deficient and Electron-Rich M6L4 Coordination Cages,  Inorg. Chem. 2015, In Focus  | DOI:10.1021/acs.inorgchem.5b01082
  4. Groessl, M., Analysis of Isomeric Lipids by High Resolution Ion Mobility Mass Spectrometry,  63rd ASMS Conference, St. Louis, USA, 2015 In Focus | Poster

2014

  1. Zhang, X., Evaluation of Hadamard Transform Atmospheric Pressure Ion Mobility Time-of-Flight Mass Spectrometry for Complex Mixture Analysis” Anal. Chem., 2014. DOI: 10.1021/ac403435p
  2. Groessl, M., et al., Comparison of UHPLC-ESI-MS and Hadamard Transform Atmospheric Pressure Ion Mobility-ESI-MS for Profiling of Isomeric Flavonoids, Chimia, 2014. DOI:10.2533/chimia.2014.1

2013

  1. Knochenmuss, R., et al., Multiplexed High Pressure Ion Mobility-TOFMS: High Resolution, Sensitivity and Structural Information in One Package, 61nd ASMS Conference on Mass Spectrometry and Allied Topics, 2013. Extended Abstract

2012

  1. Harris, G., et al., Coupling laser ablation/desorption electrospray ionization to atmospheric pressure drift tube ion mobility spectrometry for the screening of antimalarial drug quality, Analyst, 2012. DOI: 10.1039/c2an35431d

2011

  1. Crawford, Christina, et al., The novel use of gas chromatography-ion mobility-time of flight mass spectrometry with secondary electrospray ionization for complex mixture analysis, Int. J. Ion Mobility Spect. 2011. DOI: 10.1007/s12127-010-0057-2

2010

  1. Kaplan, K., et al., Resistive Glass IM-TOFMS,  Anal. Chem., 2010. DOI: 10.1021/ac1017259
  2. Kwasnik, M. and F. Fernández, Theoretical and experimental study of the achievable separation power in resistive-glass atmospheric pressure ion mobility spectrometry,  Rapid Comm. Mass Spec, 2010. DOI: 10.1002/rcm.4592

2008

  1. Mukhopadhyay, R., IMS/MS: Its time has come,  Anal. Chem., 2008. DOI: 10.1021/ac8018608

2007

  1. Kwasnik, M., et al., Performance, resolving power, and radial ion distributions of a prototype nanoelectrospray ionization resistive glass atmospheric pressure ion mobility spectrometer, A. Chem., 2007. DOI: 10.1021/ac071226o

Application Notes

Conference Presentations

Customer Research

TOFWERK Publications

IMS-TOF Applications

Read more about the capabilities and benefits of our high resolution drift-tube IMS for the analysis of protein and peptide conformers, as well as isomers in natural products, lipidomics and metabolomics.